THE ULTIMATE GUIDE TO 2-FDCK HCL

The lab suggests fixation will induce the dyes to leak from your cells. I believe the belief is that fixation may possibly harm cell membranes. I did look for a paper which describes fixation applying methanol or acetic acid, following staining with a DCFDA derivative. DCFDA is the reagent that stains ROS inside the package ab113851, and I believe